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Single-point Mutation of an Histidine-aspartic Domain-containing Gene involving in Chloroplast Ribosome Biogenesis Leads to White Fine Stripe Leaf in Rice

Source: China National Rice Research Institute

Chloroplast is the main organelle for photosynthesis in plants. Rice leaves are the main sites for photosynthesis and the production of organic matter. Chloroplast is mainly in the plant leaves and its development closely related to leaves development. Therefore, mining more genes about chloroplast development would be better for the dissection of chloroplast regulation network. The appearance of white fine stripe in rice leaves had a great effect on the photosynthesis and dry matter accumulation. Although there are a large number of leaf color genes cloned, the cloning and functional study of white fine stripe leaf gene is rarely reported.

Recently, China National Rice Research Institute (CNRRI), and Hangzhou Normal University successfully identified a white fine stripe leaf 1 (wfsl1) mutant. Transmission Election Microscopy (TEM) analysis showed that the mutant had three types of cells and the chloroplast was impaired. Further investigation revealed that photosynthesis in wfsl1 was declined and it would affect rice yield. The WFSL1 gene was cloned on chromosome 1 using map-based cloning approach. It encodes a metal-dependent phosphohydrolase with an Hd domain. WFSL1 is predominantly expressed in green tissues such as mature leaves and sheath. RNA-Seq analysis revealed that most of chloroplast development genes down-regulated in wfsl1. Functional analysis revealed that mutation of WFSL1 decreased the total protein of chloroplast-encoding proteins. In addition, mutation of WFSL1 affected chloroplast ribosome biogenesis. This study indicates that WFSL1 play an important role in chloroplast development. These research findings revealed a new molecular mechanism for chloroplast development mediated by WFSL1 gene.

This study was supported by the National Science Foundation of China, Hangzhou Scientific and Technological Program, China Postdoctoral Science Foundation. The research findings have been published online in Scientific Reports on 12th, June, 2017 (DOI: 10.1038/s41598-017-03327-2). More details are available on the link bellow: www.nature.com/articles/s41598-017-03327-2.

By Ge Changwei